Prothrombin time derived fibrinogen determination on Sysmex CA-6000

Prothrombin time derived fibrinogen determination on Sysmex CA-6000.

AIM To evaluate PT derived fibrinogen determinations with reference to the Clauss fibrinogen assay using a Sysmex CA-6000 random access coagulation analyser. METHODS Samples were analysed from normal subjects (n = 20), patients with renal or liver dysfunction (n = 25), critically ill patients (n = 25), patients receiving oral anticoagulant treatment (n = 50), and patients with a haemoglobinop...

Diagnostic utility of comparing fibrinogen Clauss and prothrombin time derived method.

A prothrombin time (PT) derived method has been described to measure plasma fibrinogen concentration. This method is not a direct determination of plasma fibrinogen but an estimation of the fibrinogen concentration from the absorbance changes at 450 nm (delta OD) during the clotting process of the PT on an automated photo optical coagulometers [1,2]. Although this assay is quick, economical and...

Influence of fibrinogen concentration upon plasma prothrombin time.

Variations in the reaction between thrombin and fibrinogen and their effect on the prothrombin time.

The appearance of fibrin provides the end-point in every clotting test, and the duration of the thrombin-fibrinogen reaction is thus a component of any measurement of clottinig time' While many tests are designed to study the early stages of clotting, in interpreting the results it is necessary to consider to what extent the observed differences may be due simply to differences in the " indicat...

Comparison of plasma with whole blood prothrombin time and fibrinogen on the same instrument.

We compared plasma with whole blood (WB) international normalized ratio (INR) and fibrinogen using the same instrument and reagents. WBINRs were 50% higher than plasma INRs. After increasing the WB sample volume 40% and adjusting the International Sensitivity Index, WBINRs were similar to plasma INRs [adjusted WBINR = 0.99(plasma INR) - 0.02; r(2) = 0.98; n = 155], but the average difference in...